Meiotic recombination in the offspring of Microbotryum hybrids and its impact on pathogenicity.
Identifieur interne : 000113 ( Main/Exploration ); précédent : 000112; suivant : 000114Meiotic recombination in the offspring of Microbotryum hybrids and its impact on pathogenicity.
Auteurs : Britta Bueker [Allemagne, États-Unis] ; Marco Alexandre Guerreiro [Allemagne] ; Michael E. Hood [États-Unis] ; Andreas Brachmann [Allemagne] ; Sven Rahmann [Allemagne] ; Dominik Begerow [Allemagne]Source :
- BMC evolutionary biology [ 1471-2148 ] ; 2020.
Abstract
BACKGROUND
Hybridization is a central mechanism in evolution, producing new species or introducing important genetic variation into existing species. In plant-pathogenic fungi, adaptation and specialization to exploit a host species are key determinants of evolutionary success. Here, we performed experimental crosses between the two pathogenic Microbotryum species, M. lychnidis-dioicae and M. silenes-acaulis that are specialized to different hosts. The resulting offspring were analyzed on phenotypic and genomic levels to describe genomic characteristics of hybrid offspring and genetic factors likely involved in host-specialization.
RESULTS
Genomic analyses of interspecific fungal hybrids revealed that individuals were most viable if the majority of loci were inherited from one species. Interestingly, species-specific loci were strictly controlled by the species' origin of the mating type locus. Moreover we detected signs of crossing over and chromosome duplications in the genomes of the analyzed hybrids. In Microbotryum, mitochondrial DNA was found to be uniparentally inherited from the a
CONCLUSION
The combination of genome analyses with experimental selection and hybridization is a promising way to investigate host-pathogen interactions. This study manifests genetic factors of host specialization that are required for successful biotrophic infection of the post-zygotic stage, but also demonstrates the strong influence of intra-genomic conflicts or instabilities on the viability of hybrids in the haploid host-independent stage.
DOI: 10.1186/s12862-020-01689-2
PubMed: 32942986
PubMed Central: PMC7499883
Affiliations:
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Hood</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Amherst College, 220 South Pleasant Street, Amherst, MA, 01002, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Amherst College, 220 South Pleasant Street, Amherst, MA, 01002</wicri:regionArea><wicri:noRegion>01002</wicri:noRegion></affiliation></author><author><name sortKey="Brachmann, Andreas" sort="Brachmann, Andreas" uniqKey="Brachmann A" first="Andreas" last="Brachmann">Andreas Brachmann</name><affiliation wicri:level="1"><nlm:affiliation>Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, 82152, Planegg- Martinsried, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, 82152, Planegg- Martinsried</wicri:regionArea><wicri:noRegion>82152, Planegg- Martinsried</wicri:noRegion><wicri:noRegion>82152, Planegg- Martinsried</wicri:noRegion><wicri:noRegion>Planegg- Martinsried</wicri:noRegion></affiliation></author><author><name sortKey="Rahmann, Sven" sort="Rahmann, Sven" uniqKey="Rahmann S" first="Sven" last="Rahmann">Sven Rahmann</name><affiliation wicri:level="1"><nlm:affiliation>Genominformatik, Institut für Humangenetik, Medizinische Fakultät, Universität Duisburg-Essen, Hufelandstraße 55, 45122, Essen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Genominformatik, Institut für Humangenetik, Medizinische Fakultät, Universität Duisburg-Essen, Hufelandstraße 55, 45122, Essen</wicri:regionArea><wicri:noRegion>45122, Essen</wicri:noRegion><wicri:noRegion>45122, Essen</wicri:noRegion><wicri:noRegion>Essen</wicri:noRegion></affiliation></author><author><name sortKey="Begerow, Dominik" sort="Begerow, Dominik" uniqKey="Begerow D" first="Dominik" last="Begerow">Dominik Begerow</name><affiliation wicri:level="1"><nlm:affiliation>AG Geobotanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780, Bochum, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>AG Geobotanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780, Bochum</wicri:regionArea><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>Bochum</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC evolutionary biology</title><idno type="eISSN">1471-2148</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Hybridization is a central mechanism in evolution, producing new species or introducing important genetic variation into existing species. In plant-pathogenic fungi, adaptation and specialization to exploit a host species are key determinants of evolutionary success. Here, we performed experimental crosses between the two pathogenic Microbotryum species, M. lychnidis-dioicae and M. silenes-acaulis that are specialized to different hosts. The resulting offspring were analyzed on phenotypic and genomic levels to describe genomic characteristics of hybrid offspring and genetic factors likely involved in host-specialization.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Genomic analyses of interspecific fungal hybrids revealed that individuals were most viable if the majority of loci were inherited from one species. Interestingly, species-specific loci were strictly controlled by the species' origin of the mating type locus. Moreover we detected signs of crossing over and chromosome duplications in the genomes of the analyzed hybrids. In Microbotryum, mitochondrial DNA was found to be uniparentally inherited from the a</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The combination of genome analyses with experimental selection and hybridization is a promising way to investigate host-pathogen interactions. This study manifests genetic factors of host specialization that are required for successful biotrophic infection of the post-zygotic stage, but also demonstrates the strong influence of intra-genomic conflicts or instabilities on the viability of hybrids in the haploid host-independent stage.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32942986</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2148</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>09</Month><Day>17</Day></PubDate></JournalIssue><Title>BMC evolutionary biology</Title><ISOAbbreviation>BMC Evol Biol</ISOAbbreviation></Journal><ArticleTitle>Meiotic recombination in the offspring of Microbotryum hybrids and its impact on pathogenicity.</ArticleTitle><Pagination><MedlinePgn>123</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12862-020-01689-2</ELocationID><Abstract><AbstractText Label="BACKGROUND">Hybridization is a central mechanism in evolution, producing new species or introducing important genetic variation into existing species. In plant-pathogenic fungi, adaptation and specialization to exploit a host species are key determinants of evolutionary success. Here, we performed experimental crosses between the two pathogenic Microbotryum species, M. lychnidis-dioicae and M. silenes-acaulis that are specialized to different hosts. The resulting offspring were analyzed on phenotypic and genomic levels to describe genomic characteristics of hybrid offspring and genetic factors likely involved in host-specialization.</AbstractText><AbstractText Label="RESULTS">Genomic analyses of interspecific fungal hybrids revealed that individuals were most viable if the majority of loci were inherited from one species. Interestingly, species-specific loci were strictly controlled by the species' origin of the mating type locus. Moreover we detected signs of crossing over and chromosome duplications in the genomes of the analyzed hybrids. In Microbotryum, mitochondrial DNA was found to be uniparentally inherited from the a<sub>2</sub> mating type. Genome comparison revealed that most gene families are shared and the majority of genes are conserved between the two species, indicating very similar biological features, including infection and pathogenicity processes. Moreover, we detected 211 candidate genes that were retained under host-driven selection of backcrossed lines. These genes and might therefore either play a crucial role in host specialization or be linked to genes that are essential for specialization.</AbstractText><AbstractText Label="CONCLUSION">The combination of genome analyses with experimental selection and hybridization is a promising way to investigate host-pathogen interactions. This study manifests genetic factors of host specialization that are required for successful biotrophic infection of the post-zygotic stage, but also demonstrates the strong influence of intra-genomic conflicts or instabilities on the viability of hybrids in the haploid host-independent stage.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bueker</LastName><ForeName>Britta</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>AG Geobotanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780, Bochum, Germany. britta.bueker@rub.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biology, Amherst College, 220 South Pleasant Street, Amherst, MA, 01002, USA. britta.bueker@rub.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guerreiro</LastName><ForeName>Marco Alexandre</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>AG Geobotanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780, Bochum, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hood</LastName><ForeName>Michael E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Department of Biology, Amherst College, 220 South Pleasant Street, Amherst, MA, 01002, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brachmann</LastName><ForeName>Andreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, 82152, Planegg- Martinsried, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahmann</LastName><ForeName>Sven</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Genominformatik, Institut für Humangenetik, Medizinische Fakultät, Universität Duisburg-Essen, Hufelandstraße 55, 45122, Essen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Begerow</LastName><ForeName>Dominik</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>AG Geobotanik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780, Bochum, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R15 GM119092</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>BE2201/7-1</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country>International</Country></Grant><Grant><GrantID>BE2201/23-1</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Evol Biol</MedlineTA><NlmUniqueID>100966975</NlmUniqueID><ISSNLinking>1471-2148</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Backcrossing</Keyword><Keyword MajorTopicYN="Y">Effectors</Keyword><Keyword MajorTopicYN="Y">Experimental selection</Keyword><Keyword MajorTopicYN="Y">Host specialization</Keyword><Keyword MajorTopicYN="Y">Host-pathogen interaction</Keyword><Keyword MajorTopicYN="Y">Hybrid speciation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>10</Day></PubMedPubDate><PubMedPubDate 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